WebJan 13, 2010 · PMA treatment enhanced the adherence of the THP-1 cells relative to untreated cells but did not induce the firm adherence of MDM (data not shown). If cells were treated with PMA and then rested by culture for a further 5 days in the absence of PMA (PMAr) they showed a much greater increase in cytoplasmic volume and more closely … WebAug 7, 2008 · 160 nM PMA (Sigma) in RPMI medium was added to differentiate the THP-1 cells for 72 h at 37°C for all the cytokine assays through this study. The criteria for the differentiation of the THP-1 cells, adherence, morphological changes, and changes in expression of the cell surface makers such as integrin, FcγRI, CD4, and MHC class II …
Standardized protocols for differentiation of THP-1 cells to
WebFor Western blot analysis, 1.6 x 10 6 THP-1 cells were PMA-differentiated into macrophages in 6-well plates (Thermo Scientific). On the next day, cells were incubated with or without the indicated inhibitors for 90 min in RPMI supplemented with 1% FCS (Sigma-Aldrich) ... WebMar 1, 2024 · However, priming THP-1 cells with 10 ng/ml PMA for 24 h and then resting for 24 h before exposing the primed cells to IL-4 for 48 h resulted in cells which transcribed MRC1 (Morón-Calvente et al., 2024), which encodes the C-type lectin mannose receptor C … chef andy co
Standardized protocols for differentiation of THP-1 cells to ...
Web5 IRF1) or alternative activation markers (CCL17, ALOX15, CCL26, TGM2, CD200R and CD206).Similar conditions can be used for polarizing PMA-primed THP-1 cells with IL-10 which result in cells that transcribe CD163, C1QA and the M(IL-10) marker gene SEPP1 31, but not MRC1. The M(IL-4) cells also express lower levels of Fc RIa and higher levels of the WebSep 26, 2024 · The THP-1 cells were incubated with 100 ng/ml PMA for 0, 1, 2 and 3 days respectively, and unattached cells in the supernatant were counted. The cell adherence rate was calculated according to the cell number of THP-1 cells without stimulation. Flow cytometry. The THP-1 cells were incubated with 100 ng/ml PMA for 0, 1, 2 and 3 days … WebNov 18, 2015 · Previous analyses have reported that the human monocytic cell line THP1 can be differentiated into cells with macrophage-like characteristics by phorbol 12 … chef and the frog whiteville