WebNov 1, 2004 · Telomere Dysfunction of EPCs Leads to Chk2 Induction Because loss of the telomere-capping protein TRF2 leads to telomere dysfunction, as measured by activation of the DNA damage protein kinase pathway, we investigated this possible downstream contender in TRF2 signaling. WebThe effect of CHK2 knockdown on TAM response in ( C) LCC2 and ( D) AK-47. LCC2 and AK-47 are TAM-resistant cell lines. The cells were incubated with 20 nM of the siRNA and 4 µM of TAM for 96 h. Cell viability was determined by MTT assay. Results are shown as mean ± SD from six independent experiments.
Chk2 Molecular Interaction Map and Rationale for Chk2 Inhibitors
WebIn human cells, following genotoxic stress, CHK2 is activated and phosphorylates >20 proteins to induce the appropriate cellular response, which, depending on the extent of damage, the cell type, and other factors, could be cell cycle checkpoint activation, induction of apoptosis or senescence, DNA repair, or tolerance of the damage. WebWhether senescence induction is an advantage or a backdrop for tumour treatment has still to be elucidated since experimental proof in human tumour models is still in an infant stage. This review focuses on the basic mechanisms and recent advances for the induction of senescence as a potential outcome of cancer therapy and discusses the ... optical to hdmi arc
WRN helicase is a synthetic lethal target in microsatellite ... - Nature
WebFeb 19, 2024 · Sustained CHK2 activity, but not ATM activity, is critical to maintain a G1 arrest after DNA damage in untransformed cells. Our data indicate that G1 checkpoint maintenance relies on CHK2 and that its negative regulation is crucial for G1 checkpoint recovery after DSB induction. WebApr 10, 2024 · a, Immunoblot. γH2AX, phospho-CHK2 (T86) and total CHK2, WRN and GAPDH levels following WRN knockout. Etoposide and hydroxyurea were used to generate DSBs and replication stress, respectively.... WebDec 1, 2009 · Phospho-CHK2 was induced, whereas Mdm-2 and Bcl-2 levels declined. These data are consistent with the induction of p53-dependent apoptotic response. In the intestine, the deletion of Elavl1 and induction of p53 and its downstream targets, PUMA and p21 Cip1/Waf1, were observed in progenitor cells of the crypts (Figure (Figure9C). 9 … optical to hdmi cable walmart